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vectastain universal quick kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectastain universal quick kit
    Vectastain Universal Quick Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 505 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/PK-8800/VECTASTAIN+Universal+Quick+HRP+Kit+(Peroxidase%2C+concentrate)/pm42798296-81-18-26
    Average 94 stars, based on 505 article reviews
    vectastain universal quick kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: SUN11602 Accelerates Early Peripheral Nerve Regeneration in an Experimental Median Nerve Transection Model.
    Article Snippet: Tissues were then blocked with normal horse serum for 10 min, followed by incubation with primary anti- IBA- 1 (1:100; sc- 32725; Santa Cruz Biotechnology) at room temperature for 3 h. Next, nloaded from https://onlinelibrary.w iley.com /doi/10.1111/jcm m .71375 by R obson Souza Serralha - U niversity O f Sao Paulo - B razil , on [30/09/2026] .. C reative C ons L icense 4 of 11 Journal of Cellular and Molecular Medicine, 2026 using the VECTASTAIN Universal Quick Kit, Peroxidase, R.T.U. (PK- 7800; Vector Laboratories, Burlingame, CA, USA), the sections were incubated with a secondary antibody after being cleaned with PBS ..

    Article Title: Neuroprotective Role of Cannabinoid CB 1 and GPR55 Receptors in a Cell Model of Multiple Sclerosis
    Article Snippet: For CB 1 R detection, cells were incubated overnight at 4 °C in a humidified chamber with a rabbit anti-CB 1 R antibody (1:500; PA1-745, Thermo Scientific, Rockford, USA). .. After three PBS washes, cells were incubated for 30 min at room temperature with a biotinylated horse universal secondary antibody (1:40; Vector, PK-8800, Vector Laboratories Inc., Newark, NJ, USA), followed by streptavidin Alexa Fluor ® 550 conjugate (1:500; S2138, Invitrogen, Paisley, Scotland, UK). ..

    Article Title: Histopathological alterations in airways associated with physiological changes in airway allergy phenotypes
    Article Snippet: .. Vectastain Universal Quick Kit (Vector Laboratories, Inc., Burlingame, CA, USA), following the sequential incubation steps of blocking serum, secondary antibody, and streptavidin/peroxidase complex. .. The chromogen used was 3-amino-9-ethylcarbazole (BioGenex, Fremont, CA, USA), and the sections were counterstained with Mayer's hematoxylin.

    Article Title: Neuroprotective Role of Cannabinoid CB 1 and GPR55 Receptors in a Cell Model of Multiple Sclerosis.
    Article Snippet: For CB1R detection, cells were incubated overnight at 4 °C in a humidified chamber with a rabbit anti-CB1R antibody (1:500; PA1-745, Thermo Scientific, Rockford, USA). .. After three PBS washes, cells were incubated for 30 min at room temperature with a biotinylated horse universal secondary antibody (1:40; Vector, PK-8800, Vector Laboratories Inc., Newark, NJ, USA), followed by streptavidin Alexa Fluor® 550 conjugate (1:500; S2138, Invitrogen, Paisley, Scotland, UK). ..

    Article Title: Annexin A1 and its N-terminal peptide Ac2-26 regulate dopaminergic degeneration and neuroinflammation in a 6-OHDA model of Parkinson’s disease
    Article Snippet: For immunohistochemical analysis, coronal free-floating sections were incubated for 2 h with a biotinylated horse anti-mouse secondary antibody (cat.#PK-7800, VECTASTAIN® Universal Quick HRP Kit; Vector Laboratories, Newark, CA, USA). .. After further washing in PBS, sections were incubated for 1 h in an avidin-biotin-peroxidase complex (VECTASTAIN® Universal Quick HRP Kit; Vector Laboratories). ..

    SYBR Green Assay:

    Article Title: Comparative 28-day mouse study of topically applied aryl hydrocarbon receptor ligands: microbiota-derived indoles, therapeutic tapinarof, pollutants 2,3,7,8-tetrachlorodibenzo- p -dioxin and diesel exhaust particles
    Article Snippet: .. The following chemicals and reagents were purchased: dimethyl sulfoxide (DMSO) (Sigma-Aldrich, D2650, Milwaukee, WI, USA), TAP (MedChemExpress, HY109044 , Monmouth Junction, NJ, USA), IPY (Sigma-Aldrich, I7017, St. Louis, MO, USA), IAld (Sigma-Aldrich, 129445–5G, St. Louis, MO, USA), TCDD (Cambridge Isotope Laboratories, ED-901-B, Tewksbury, MA, USA), propylene glycol (Sigma-Aldrich, P4347, St. Louis, MO, USA), DEP (Sigma-Aldrich, NIST1650b, St. Louis, MO, USA), RNA STAT-60 (TEL-TEST INC., CS-111, Friendswood, TX, USA), phenol:chloroform:isoamyl alcohol 25:24:1, (Invitrogen, 15513047, Carlsbad, CA, USA), DNase I (New England Biolabs, M0303L, Ipswich, MA, USA), iTaq Universal SYBR Green Supermix (Bio-Rad, 1725124, Richmond, CA, USA), MMLV reverse transcriptase (Invitrogen, 28025–021, Carlsbad, CA, USA), RNAseOUT (Invitrogen, 10777019, Carlsbad, CA, USA), anti-CYP1A1 antibody (Santa Cruz Biotechnology, sc-25304, Dallas, TX, USA), VECTASTAIN Universal Quick HRP Kit (Vector Laboratories, PK-8800, Newark, CA, USA), diaminobenzidine (DAB) substrate kit (Vector Laboratories, SK-4100, Newark, CA, USA), Tissue-Tek optimal cutting temperature (OCT) compound (Sakura, 4583, Torrance, CA, USA), Oil Red O Isopropanol Solution (Electron Microscopy Science, 26503–02), Mayer’s Hematoxylin (Sigma-Aldrich, CM393, St. Louis, MO, USA), anti-filaggrin (FLG) antibody (Thermo Fisher Scientific, Rockford, MA5–13440, IL USA), anti-epigen (EPGN) antibody (Boster Biological Technology, A10011, Pleasanton CA, USA), Aqua Mount (Lerner Laboratories, 13800, Northbrook, IL, USA), isoflurane (Covetrus, 11695067772, Fort Worth, TX, USA). .. Eight-week old male and female C57BL/6J (JAX# 000664) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and caged under standard temperature (18–2600B0030C) and light-dark cycle (12 h-12 h) with a humidity range of 40–60%.

    Article Title: Comparative 28-day mouse study of topically applied aryl hydrocarbon receptor ligands: microbiota-derived indoles, therapeutic tapinarof, pollutants 2,3,7,8-tetrachlorodibenzo-p-dioxin and diesel exhaust particles.
    Article Snippet: The aryl hydrocarbon receptor (AHR) regulates skin responses to microbial, therapeutic, and pollutant ligands.. While AHR activation has been linked to both the etiology and treatment of skin diseases, the comparative effects of distinct ligand classes remain understudied.. Here we evaluated systemic and cutaneous effects of different AHR ligands applied topically for 28 days.

    Reverse Transcription:

    Article Title: Comparative 28-day mouse study of topically applied aryl hydrocarbon receptor ligands: microbiota-derived indoles, therapeutic tapinarof, pollutants 2,3,7,8-tetrachlorodibenzo- p -dioxin and diesel exhaust particles
    Article Snippet: .. The following chemicals and reagents were purchased: dimethyl sulfoxide (DMSO) (Sigma-Aldrich, D2650, Milwaukee, WI, USA), TAP (MedChemExpress, HY109044 , Monmouth Junction, NJ, USA), IPY (Sigma-Aldrich, I7017, St. Louis, MO, USA), IAld (Sigma-Aldrich, 129445–5G, St. Louis, MO, USA), TCDD (Cambridge Isotope Laboratories, ED-901-B, Tewksbury, MA, USA), propylene glycol (Sigma-Aldrich, P4347, St. Louis, MO, USA), DEP (Sigma-Aldrich, NIST1650b, St. Louis, MO, USA), RNA STAT-60 (TEL-TEST INC., CS-111, Friendswood, TX, USA), phenol:chloroform:isoamyl alcohol 25:24:1, (Invitrogen, 15513047, Carlsbad, CA, USA), DNase I (New England Biolabs, M0303L, Ipswich, MA, USA), iTaq Universal SYBR Green Supermix (Bio-Rad, 1725124, Richmond, CA, USA), MMLV reverse transcriptase (Invitrogen, 28025–021, Carlsbad, CA, USA), RNAseOUT (Invitrogen, 10777019, Carlsbad, CA, USA), anti-CYP1A1 antibody (Santa Cruz Biotechnology, sc-25304, Dallas, TX, USA), VECTASTAIN Universal Quick HRP Kit (Vector Laboratories, PK-8800, Newark, CA, USA), diaminobenzidine (DAB) substrate kit (Vector Laboratories, SK-4100, Newark, CA, USA), Tissue-Tek optimal cutting temperature (OCT) compound (Sakura, 4583, Torrance, CA, USA), Oil Red O Isopropanol Solution (Electron Microscopy Science, 26503–02), Mayer’s Hematoxylin (Sigma-Aldrich, CM393, St. Louis, MO, USA), anti-filaggrin (FLG) antibody (Thermo Fisher Scientific, Rockford, MA5–13440, IL USA), anti-epigen (EPGN) antibody (Boster Biological Technology, A10011, Pleasanton CA, USA), Aqua Mount (Lerner Laboratories, 13800, Northbrook, IL, USA), isoflurane (Covetrus, 11695067772, Fort Worth, TX, USA). .. Eight-week old male and female C57BL/6J (JAX# 000664) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and caged under standard temperature (18–2600B0030C) and light-dark cycle (12 h-12 h) with a humidity range of 40–60%.

    Article Title: Comparative 28-day mouse study of topically applied aryl hydrocarbon receptor ligands: microbiota-derived indoles, therapeutic tapinarof, pollutants 2,3,7,8-tetrachlorodibenzo-p-dioxin and diesel exhaust particles.
    Article Snippet: The aryl hydrocarbon receptor (AHR) regulates skin responses to microbial, therapeutic, and pollutant ligands.. While AHR activation has been linked to both the etiology and treatment of skin diseases, the comparative effects of distinct ligand classes remain understudied.. Here we evaluated systemic and cutaneous effects of different AHR ligands applied topically for 28 days.

    Electron Microscopy:

    Article Title: Comparative 28-day mouse study of topically applied aryl hydrocarbon receptor ligands: microbiota-derived indoles, therapeutic tapinarof, pollutants 2,3,7,8-tetrachlorodibenzo- p -dioxin and diesel exhaust particles
    Article Snippet: .. The following chemicals and reagents were purchased: dimethyl sulfoxide (DMSO) (Sigma-Aldrich, D2650, Milwaukee, WI, USA), TAP (MedChemExpress, HY109044 , Monmouth Junction, NJ, USA), IPY (Sigma-Aldrich, I7017, St. Louis, MO, USA), IAld (Sigma-Aldrich, 129445–5G, St. Louis, MO, USA), TCDD (Cambridge Isotope Laboratories, ED-901-B, Tewksbury, MA, USA), propylene glycol (Sigma-Aldrich, P4347, St. Louis, MO, USA), DEP (Sigma-Aldrich, NIST1650b, St. Louis, MO, USA), RNA STAT-60 (TEL-TEST INC., CS-111, Friendswood, TX, USA), phenol:chloroform:isoamyl alcohol 25:24:1, (Invitrogen, 15513047, Carlsbad, CA, USA), DNase I (New England Biolabs, M0303L, Ipswich, MA, USA), iTaq Universal SYBR Green Supermix (Bio-Rad, 1725124, Richmond, CA, USA), MMLV reverse transcriptase (Invitrogen, 28025–021, Carlsbad, CA, USA), RNAseOUT (Invitrogen, 10777019, Carlsbad, CA, USA), anti-CYP1A1 antibody (Santa Cruz Biotechnology, sc-25304, Dallas, TX, USA), VECTASTAIN Universal Quick HRP Kit (Vector Laboratories, PK-8800, Newark, CA, USA), diaminobenzidine (DAB) substrate kit (Vector Laboratories, SK-4100, Newark, CA, USA), Tissue-Tek optimal cutting temperature (OCT) compound (Sakura, 4583, Torrance, CA, USA), Oil Red O Isopropanol Solution (Electron Microscopy Science, 26503–02), Mayer’s Hematoxylin (Sigma-Aldrich, CM393, St. Louis, MO, USA), anti-filaggrin (FLG) antibody (Thermo Fisher Scientific, Rockford, MA5–13440, IL USA), anti-epigen (EPGN) antibody (Boster Biological Technology, A10011, Pleasanton CA, USA), Aqua Mount (Lerner Laboratories, 13800, Northbrook, IL, USA), isoflurane (Covetrus, 11695067772, Fort Worth, TX, USA). .. Eight-week old male and female C57BL/6J (JAX# 000664) mice were purchased from Jackson Laboratory (Bar Harbor, ME, USA) and caged under standard temperature (18–2600B0030C) and light-dark cycle (12 h-12 h) with a humidity range of 40–60%.

    Article Title: Comparative 28-day mouse study of topically applied aryl hydrocarbon receptor ligands: microbiota-derived indoles, therapeutic tapinarof, pollutants 2,3,7,8-tetrachlorodibenzo-p-dioxin and diesel exhaust particles.
    Article Snippet: The aryl hydrocarbon receptor (AHR) regulates skin responses to microbial, therapeutic, and pollutant ligands.. While AHR activation has been linked to both the etiology and treatment of skin diseases, the comparative effects of distinct ligand classes remain understudied.. Here we evaluated systemic and cutaneous effects of different AHR ligands applied topically for 28 days.

    Immunohistochemistry:

    Article Title: Tubuloside A attenuates sepsis-induced acute lung injury by modulating the NF-κB p50-Nrf2/GPX4 axis to suppress inflammation, oxidative stress and ferroptosis.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Staining:

    Article Title: Tubuloside A attenuates sepsis-induced acute lung injury by modulating the NF-κB p50-Nrf2/GPX4 axis to suppress inflammation, oxidative stress and ferroptosis.
    Article Snippet: This is a PDF of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability.. This version will undergo additional copyediting, typesetting and review before it is published in its final form.. As such, this version is no longer the Accepted Manuscript, but it is not yet the definitive Version of Record; we are providing this early version to give early visibility of the article.

    Blocking Assay:

    Article Title: Histopathological alterations in airways associated with physiological changes in airway allergy phenotypes
    Article Snippet: .. Vectastain Universal Quick Kit (Vector Laboratories, Inc., Burlingame, CA, USA), following the sequential incubation steps of blocking serum, secondary antibody, and streptavidin/peroxidase complex. .. The chromogen used was 3-amino-9-ethylcarbazole (BioGenex, Fremont, CA, USA), and the sections were counterstained with Mayer's hematoxylin.

    Avidin-Biotin Assay:

    Article Title: Annexin A1 and its N-terminal peptide Ac2-26 regulate dopaminergic degeneration and neuroinflammation in a 6-OHDA model of Parkinson’s disease
    Article Snippet: For immunohistochemical analysis, coronal free-floating sections were incubated for 2 h with a biotinylated horse anti-mouse secondary antibody (cat.#PK-7800, VECTASTAIN® Universal Quick HRP Kit; Vector Laboratories, Newark, CA, USA). .. After further washing in PBS, sections were incubated for 1 h in an avidin-biotin-peroxidase complex (VECTASTAIN® Universal Quick HRP Kit; Vector Laboratories). ..



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